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+NOTE: After the coupling of the first C-terminal amino acid of all [peptides](https://namastenaukri.com/employer/ipdz/) to the amino group of the membrane, the amino groups between the spots and also a few of the amino groups inside the spot areas don't kind bonds with the amino acids. Be certain that all of the purification buffers are at four °C and carry out the protein purification within the chilly room to avoid protein denaturation. WORD: This avoids the coupling of amino acids within the additional cycles with the membrane as an alternative of the growing peptide chain.
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